rabbit polyclonal α cep152 Search Results


rabbit polyclonal α cep152  (Proteintech)
93
Proteintech rabbit polyclonal α cep152
Rabbit Polyclonal α Cep152, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal α cep152/product/Proteintech
Average 93 stars, based on 1 article reviews
rabbit polyclonal α cep152 - by Bioz Stars, 2026-02
93/100 stars
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rabbit polyclonal α cep135  (Proteintech)
93
Proteintech rabbit polyclonal α cep135
Rabbit Polyclonal α Cep135, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal α cep135/product/Proteintech
Average 93 stars, based on 1 article reviews
rabbit polyclonal α cep135 - by Bioz Stars, 2026-02
93/100 stars
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rabbit α cp110  (Proteintech)
95
Proteintech rabbit α cp110
A. qRT-PCR analysis for Plk4, Plk2 and Plk1 mRNA expression in FACS-sorted cells of the bone marrow: Lin-Sca1+c-Kit+ (LSK), common lymphoid progenitor (CLP), pro B, large and small pre B and immature IgM+ B cells; spleen: transitional (T B) B cells, mature follicular (FO B) B cells, marginal zone (MZ B) B cells, CD4 and CD8 naive, effector memory (EM) and central memory (CM) T cells; thymus: double negative (DN) and double positive (DP) thymocytes; and the peritoneum: B1 B cells (B1). B. Percentage of counted cells with >4 centrioles. Centriole number was determined by fluorescence microscopy with ɣ-Tubulin, <t>CP110</t> antibody staining and Hoechst nuclear staining. C. Correlation of fraction of cells with >4 centrioles with fraction of cells in S or G2/M phase of cell cycle (SG2M), which was determined by flow cytometric analysis of mice expressing a FUCCI reporter system. Data are shown as mean of 3 individual mice. D. Immunofluorescence images and quantification of FACS-sorted large pre B cells, expressing CENT1-GFP positive daugther centrioles and stained mother centrioles with CEP164. Scale bar, 5µm. Data are shown as mean ± SD; n=3-6.
Rabbit α Cp110, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit α cp110/product/Proteintech
Average 95 stars, based on 1 article reviews
rabbit α cp110 - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier

Image Search Results


A. qRT-PCR analysis for Plk4, Plk2 and Plk1 mRNA expression in FACS-sorted cells of the bone marrow: Lin-Sca1+c-Kit+ (LSK), common lymphoid progenitor (CLP), pro B, large and small pre B and immature IgM+ B cells; spleen: transitional (T B) B cells, mature follicular (FO B) B cells, marginal zone (MZ B) B cells, CD4 and CD8 naive, effector memory (EM) and central memory (CM) T cells; thymus: double negative (DN) and double positive (DP) thymocytes; and the peritoneum: B1 B cells (B1). B. Percentage of counted cells with >4 centrioles. Centriole number was determined by fluorescence microscopy with ɣ-Tubulin, CP110 antibody staining and Hoechst nuclear staining. C. Correlation of fraction of cells with >4 centrioles with fraction of cells in S or G2/M phase of cell cycle (SG2M), which was determined by flow cytometric analysis of mice expressing a FUCCI reporter system. Data are shown as mean of 3 individual mice. D. Immunofluorescence images and quantification of FACS-sorted large pre B cells, expressing CENT1-GFP positive daugther centrioles and stained mother centrioles with CEP164. Scale bar, 5µm. Data are shown as mean ± SD; n=3-6.

Journal: bioRxiv

Article Title: Exact centriole counts are critical for B cell development but not function

doi: 10.1101/2024.02.14.580240

Figure Lengend Snippet: A. qRT-PCR analysis for Plk4, Plk2 and Plk1 mRNA expression in FACS-sorted cells of the bone marrow: Lin-Sca1+c-Kit+ (LSK), common lymphoid progenitor (CLP), pro B, large and small pre B and immature IgM+ B cells; spleen: transitional (T B) B cells, mature follicular (FO B) B cells, marginal zone (MZ B) B cells, CD4 and CD8 naive, effector memory (EM) and central memory (CM) T cells; thymus: double negative (DN) and double positive (DP) thymocytes; and the peritoneum: B1 B cells (B1). B. Percentage of counted cells with >4 centrioles. Centriole number was determined by fluorescence microscopy with ɣ-Tubulin, CP110 antibody staining and Hoechst nuclear staining. C. Correlation of fraction of cells with >4 centrioles with fraction of cells in S or G2/M phase of cell cycle (SG2M), which was determined by flow cytometric analysis of mice expressing a FUCCI reporter system. Data are shown as mean of 3 individual mice. D. Immunofluorescence images and quantification of FACS-sorted large pre B cells, expressing CENT1-GFP positive daugther centrioles and stained mother centrioles with CEP164. Scale bar, 5µm. Data are shown as mean ± SD; n=3-6.

Article Snippet: The following antibodies were used to perform immunofluorescence staining in murine cells: rabbit polyclonal α-CEP152 1:1000 (homemade, LoMastro et al ., 2022 , 1:1000), goat polyclonal α-ɣ-Tubulin (homemade, Levine et al ., 2017 , 1:1000), rabbit polyclonal α-CEP135 (homemade, LoMastro et al ., 2022 , 1:1000), mouse α-ɣ-Tubulin (Sigma-Aldrich, 1:250); rabbit α-CP110 (Protein Tech, 12780-1-AP, 1:500), goat α-mouse IgG AF568 (Thermo Fisher, A11031, 1:1000), goat α-rabbit IgG AF488 (Thermo Fisher, A-11034, 1:1000).

Techniques: Quantitative RT-PCR, Expressing, Fluorescence, Microscopy, Staining, Immunofluorescence

A-C. Pro B cells were FACS-sorted from mice of the indicated genotypes and flow cytometry was used to determine the fraction of (A) mitotic (pH3+) cells, (B) cells with DNA double strand breaks (ɣH2AX+) and (C) of dead cells (subG1) after sorting (d0) or in culture after 3, 5 and 7 days (d3, d5, d7) with IL7. D. Percentage of counted cells with more than 4 centrioles, determined by immunofluorescence with ɣ-Tubulin and CP110 antibody staining and Hoechst nuclear staining. Data are shown as mean ± SD; wild type wt (n=4-6), Pidd1 -/- (n=4) , p21 -/- (n=3), p53 -/- (n=5), BCL2 tg (n=4); *p< 0.05, **p< 0.01, ***p< 0.001, ****p< 0.0001; Genotypes were compared to wt by Two-way-ANOVA Tukey’s multiple comparisons test.

Journal: bioRxiv

Article Title: Exact centriole counts are critical for B cell development but not function

doi: 10.1101/2024.02.14.580240

Figure Lengend Snippet: A-C. Pro B cells were FACS-sorted from mice of the indicated genotypes and flow cytometry was used to determine the fraction of (A) mitotic (pH3+) cells, (B) cells with DNA double strand breaks (ɣH2AX+) and (C) of dead cells (subG1) after sorting (d0) or in culture after 3, 5 and 7 days (d3, d5, d7) with IL7. D. Percentage of counted cells with more than 4 centrioles, determined by immunofluorescence with ɣ-Tubulin and CP110 antibody staining and Hoechst nuclear staining. Data are shown as mean ± SD; wild type wt (n=4-6), Pidd1 -/- (n=4) , p21 -/- (n=3), p53 -/- (n=5), BCL2 tg (n=4); *p< 0.05, **p< 0.01, ***p< 0.001, ****p< 0.0001; Genotypes were compared to wt by Two-way-ANOVA Tukey’s multiple comparisons test.

Article Snippet: The following antibodies were used to perform immunofluorescence staining in murine cells: rabbit polyclonal α-CEP152 1:1000 (homemade, LoMastro et al ., 2022 , 1:1000), goat polyclonal α-ɣ-Tubulin (homemade, Levine et al ., 2017 , 1:1000), rabbit polyclonal α-CEP135 (homemade, LoMastro et al ., 2022 , 1:1000), mouse α-ɣ-Tubulin (Sigma-Aldrich, 1:250); rabbit α-CP110 (Protein Tech, 12780-1-AP, 1:500), goat α-mouse IgG AF568 (Thermo Fisher, A11031, 1:1000), goat α-rabbit IgG AF488 (Thermo Fisher, A-11034, 1:1000).

Techniques: Flow Cytometry, Immunofluorescence, Staining

A. Pro B cells were FACS-sorted from the indicated genotypes and put in culture. Doxycyclin was added after 48h in culture. qRT-PCR analysis of PLK4 mRNA expression in cultured pro B cells (n=3 for each genotype). B. Fraction of counted cells with more than 4 CP110 foci was determined by immunofluorescence. (n=5-6 rtTA , n=4 rtTA PLK4 ) C-D. Fraction of cells in in subG1 gate (cell death) was determined by flow cytometric cell cycle analysis. (n=6 rtTA , n=8 rtTA Plk4 , n=7 rtTA BCL2 tg , n=3 rtTA Plk4 BCL2 tg and rtTA PLK4 Pidd1 -/- ; Genotypes were compared to rtTA Plk4 or rtTA BCL2 tg for statistical analysis). E. Fraction of counted cells with more than 4 CP110 foci was determined by immunofluorescence. (n=5-6 rtTA , n=4 rtTA Plk4 , n=3-4 rtTA BCL2 tg , n=3 rtTA Plk4 BCL2 tg and rtTA PLK4 Pidd1 -/ ) F. Immunofluorescence with CP110, ɣ-Tubulin of FACS-sorted B cells was used to determine the fraction of cells with more than 4 centrioles of wt (n=4), BCL2 tg (n=3) and p53 -/- (n=3) animals. Bone marrow: pro B, large pre B, small pre B and immature IgM+ (imm. IgM) B cells; Spleen: transitional (T B), mature follicular (FO B) and marginal zone (MZ B) B cells. Data are shown as mean ± SD; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001; Two-way-ANOVA Tukey’s multiple comparisons test.

Journal: bioRxiv

Article Title: Exact centriole counts are critical for B cell development but not function

doi: 10.1101/2024.02.14.580240

Figure Lengend Snippet: A. Pro B cells were FACS-sorted from the indicated genotypes and put in culture. Doxycyclin was added after 48h in culture. qRT-PCR analysis of PLK4 mRNA expression in cultured pro B cells (n=3 for each genotype). B. Fraction of counted cells with more than 4 CP110 foci was determined by immunofluorescence. (n=5-6 rtTA , n=4 rtTA PLK4 ) C-D. Fraction of cells in in subG1 gate (cell death) was determined by flow cytometric cell cycle analysis. (n=6 rtTA , n=8 rtTA Plk4 , n=7 rtTA BCL2 tg , n=3 rtTA Plk4 BCL2 tg and rtTA PLK4 Pidd1 -/- ; Genotypes were compared to rtTA Plk4 or rtTA BCL2 tg for statistical analysis). E. Fraction of counted cells with more than 4 CP110 foci was determined by immunofluorescence. (n=5-6 rtTA , n=4 rtTA Plk4 , n=3-4 rtTA BCL2 tg , n=3 rtTA Plk4 BCL2 tg and rtTA PLK4 Pidd1 -/ ) F. Immunofluorescence with CP110, ɣ-Tubulin of FACS-sorted B cells was used to determine the fraction of cells with more than 4 centrioles of wt (n=4), BCL2 tg (n=3) and p53 -/- (n=3) animals. Bone marrow: pro B, large pre B, small pre B and immature IgM+ (imm. IgM) B cells; Spleen: transitional (T B), mature follicular (FO B) and marginal zone (MZ B) B cells. Data are shown as mean ± SD; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001; Two-way-ANOVA Tukey’s multiple comparisons test.

Article Snippet: The following antibodies were used to perform immunofluorescence staining in murine cells: rabbit polyclonal α-CEP152 1:1000 (homemade, LoMastro et al ., 2022 , 1:1000), goat polyclonal α-ɣ-Tubulin (homemade, Levine et al ., 2017 , 1:1000), rabbit polyclonal α-CEP135 (homemade, LoMastro et al ., 2022 , 1:1000), mouse α-ɣ-Tubulin (Sigma-Aldrich, 1:250); rabbit α-CP110 (Protein Tech, 12780-1-AP, 1:500), goat α-mouse IgG AF568 (Thermo Fisher, A11031, 1:1000), goat α-rabbit IgG AF488 (Thermo Fisher, A-11034, 1:1000).

Techniques: Quantitative RT-PCR, Expressing, Cell Culture, Immunofluorescence, Cell Cycle Assay

A. FACS-sorted pro B cells of wt (n=4), p21 -/- (n=4), p53 -/- (n=4) and BCL2 tg (n=3) were treated with 25, 125 or 200nM centrinone for 3 days in presence of IL-7. Centriole distribution and (B) depletion (<2 centrioles) was assessed by immunofluorescence with ɣ-Tubulin, CP110 antibodies and Hoechst staining. C. Fraction of cells in G1-, S-, G2/M-phase of the cell cycle and (D) fraction of cells in subG1 gate (cell death) was determined by flow cytometric cell cycle analysis. Data are shown as mean ± SD; n=3-5; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001; Genotypes were compared to wt by Two-way-ANOVA Tukey’s multiple comparisons test.

Journal: bioRxiv

Article Title: Exact centriole counts are critical for B cell development but not function

doi: 10.1101/2024.02.14.580240

Figure Lengend Snippet: A. FACS-sorted pro B cells of wt (n=4), p21 -/- (n=4), p53 -/- (n=4) and BCL2 tg (n=3) were treated with 25, 125 or 200nM centrinone for 3 days in presence of IL-7. Centriole distribution and (B) depletion (<2 centrioles) was assessed by immunofluorescence with ɣ-Tubulin, CP110 antibodies and Hoechst staining. C. Fraction of cells in G1-, S-, G2/M-phase of the cell cycle and (D) fraction of cells in subG1 gate (cell death) was determined by flow cytometric cell cycle analysis. Data are shown as mean ± SD; n=3-5; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001; Genotypes were compared to wt by Two-way-ANOVA Tukey’s multiple comparisons test.

Article Snippet: The following antibodies were used to perform immunofluorescence staining in murine cells: rabbit polyclonal α-CEP152 1:1000 (homemade, LoMastro et al ., 2022 , 1:1000), goat polyclonal α-ɣ-Tubulin (homemade, Levine et al ., 2017 , 1:1000), rabbit polyclonal α-CEP135 (homemade, LoMastro et al ., 2022 , 1:1000), mouse α-ɣ-Tubulin (Sigma-Aldrich, 1:250); rabbit α-CP110 (Protein Tech, 12780-1-AP, 1:500), goat α-mouse IgG AF568 (Thermo Fisher, A11031, 1:1000), goat α-rabbit IgG AF488 (Thermo Fisher, A-11034, 1:1000).

Techniques: Immunofluorescence, Staining, Cell Cycle Assay